| Abstract: | A sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the simultaneous determination of the tranquilizers (chlorpromazine, promethazine, diazepam, azaperone, and its metabolite, azaperol) and a β-blocker (carazolol) in edible swine tissues. Sample was subjected to extract with acetonitrile, clean up by Oasis HLB solid phase extraction cartridge, and then analyzed by LC-MS-MS in multiple reaction monitoring positive ionization mode. The matrix-matched calibration curves were linear within the dynamic range of each analyte with a correlation coefficient higher than 0.99. The average recoveries of tranquilizers and carazolol spiked at three levels ranged from 74.2% to 91.8% with the relative standard deviation below 15%. The limits of detection were between 0.06 and 0.1 µg kg?1 and the limits of quantification were between 0.2 and 0.4 µg kg?1 for all analytes in swine muscle, liver, and kidney. |
