Analysis of Chlorpyrifos in Water,Fruit Juice,and Honeybee Extract by Chemiluminescent Elisa

Abstract

The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection-based immobilized antigen (indirect assay) for rapid and accurate determination of chlorpyrifos in various food matrices was tested. The limit of detection (LOD) values were 1–1.75 ng mL^?1, the standard curve midpoint (IC₅₀) was 3.5 ng mL^?1, and the assay duration was 1.5 h. Assay application to the analysis of honeybee extract resulted in chlorpyrifos recoveries varying between 62 and 83% in 5–15 ng mL^?1 herbicide concentration range.