Separation and Determination of Magnolol and Honokiol in Crude and Burn-Fried Magnolia officinalis

Abstract

The application of capillary electrophoresis (CE) to the separation and determination of the two active ingredients, magnolol and honokiol, in Magnolia officinalis and its processed product was described. Optimum separation was achieved with a fused-silica capillary tube(60.5cm × 75μm I.D.) and a Na₂B₄O₇-NaH₂PO₄ buffer(20 mmol/L) at pH=9.0 containing 20% of methanol. The applied voltage was 20 kV and the capillary thermostating temperature was kept constant at 25 deg;C.