Certification and uncertainty evaluation of the certified reference materials of poly(ethylene glycol) for molecular mass fractions by using supercritical fluid chromatography |
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Authors: | Kayori Takahashi Kana Kishine Shigetomo Matsuyama Takeshi Saito Haruhisa Kato Shinichi Kinugasa |
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Institution: | (1) Materials Characterization Division, National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1–1–1 Higashi, Tsukuba, Ibaraki 305–8565, Japan |
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Abstract: | Poly(ethylene glycol) (PEG) is a useful water-soluble polymer that has attracted considerable interest in medical and biological
science applications as well as in polymer physics. Through the use of a well-calibrated evaporative light-scattering detector
coupled with high performance supercritical fluid chromatography, we are able to determine exactly not only the average mass
but also all of the molecular mass fractions of PEG samples needed for certified reference materials issued by the National
Metrology Institute of Japan. In addition, experimental uncertainty was determined in accordance with the Guide to the expression of uncertainty in measurement (GUM). This reference material can be used to calibrate measuring instruments, to control measurement precision, and to confirm
the validity of measurement methods when determining molecular mass distributions and average molecular masses. Especially,
it is suitable for calibration against both masses and intensities for matrix-assisted laser desorption/ionization time-of-flight
mass spectrometry.
Figure Comparison between the molecular mass fractions of PEG 1000 before calibration (si) (○) and after calibration (wi) (⧫). The error bar shows the expanded uncertainty of k = 2 of each mass fraction |
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Keywords: | Certified reference material Poly(ethylene glycol) Uncertainty Evaporative light-scattering detector Supercritical fluid chromatography Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
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