UV-B induced keratinocyte apoptosis is blocked by 2-selenium-bridged beta-cyclodextrin,a GPX mimic |
| |
Authors: | Mu Ying Lv Shaowu Ren Xiaojun Jin Guanghui Liu Junqiu Yan Ganglin Li Weijia Shen Jiacong Luo Guimin |
| |
Institution: | Key Laboratory for Molecular Enzymology, Jilin University, 125 Jiefang Road, Changchun 130023, PR China. |
| |
Abstract: | Cell proliferation and cell death of keratinocytes are tightly regulated to ensure epidermal homeostasis. UV-B induces keratinocyte apoptosis. UV-B also induces lipid peroxidation of keratinocytes to increase their amount of malondialdehyde (MDA). These phenomena can be explained by the production of reactive oxygen species (ROS) induced by UV-B radiation. We synthesized 2-selenium-bridged beta-cyclodextrin (2-SeCD) to imitate glutathione peroxidase (GPX), an important antioxidant and established a damage system, in which keratinocytes can be damaged by Ultraviolet B (UV-B) radiation. Using this damage system we studied 2-SeCD protection of keratinocytes against injury induced by UV-B. Experimental results showed that 2-SeCD could protect keratinocytes from apoptosis. Moreover, 2-SeCD inhibits lipid peroxidation of keratinocytes and scavenges ROS. 2-SeCD inhibits the UV-B induced apoptotic signal transduction. This antiapoptotic mechanism may be partly related to the elimination of hydrogen peroxide. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|