Anti-fouling poly(2-hydoxyethyl methacrylate) surface coatings with specific bacteria recognition capabilities |
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Authors: | Bechir Mrabet Minh Ngoc Nguyen Aymen Majbri Samia Mahouche Mireille Turmine Amina Bakhrouf Mohamed M. Chehimi |
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Affiliation: | a Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits (LATVPEP), Faculté de Pharmacie de Monastir, 5000 Monastir, Tunisia b ITODYS, Université Paris Diderot and CNRS (UMR 7086), Bâtiment Lavoisier, 15 rue Jean de Baïf, 75205 Paris Cedex 13, France c Laboratoire d’Electrochimie et de Chimie Analytique, Université Pierre et Marie Curie and CNRS (UMR 7575), 4 place Jussieu 75005 Paris, France |
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Abstract: | Poly(2-hydroxyethyl methacrylate), PHEMA, brushes were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) on silanized glass slides bearing grafted initiators. High resolution X-ray photoelectron spectroscopy (XPS) highlighted the surface chemical changes of the glass slides upon silanization and surface-confined ATRP of HEMA. Particularly, the initiator sites from the silane were detected by their bromine Br3d core electron peak whilst the O/C atomic ratios and the high resolution C1s region of the glass–PHEMA hybrids are comparable to those of pure PHEMA, thus confirming that the PHEMA chains have indeed attached to the surface. The glass–PHEMA hybrids were found to behave as anti-fouling ultrathin coatings as they resisted non-specific Salmonella typhimurium bacterial adhesion. This behaviour is driven by the hydrophilic properties of the glass–PHEMA hybrids which were assessed by contact angle measurements. In contrast, after activation of PHEMA brushes by S. typhimurium antibodies through the trichlorotriazine coupling procedure, the bacteria specifically and strongly attached to the PHEMA-coated glass slides as judged from optical microscope observation. |
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Keywords: | Bacteria PHEMA ATRP Anti-fouling Bioadhesion |
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