| 基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究 |
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| 引用本文： | 汪红梅,林丽清,翁少煌,王丽满,刘银环,林新华.基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究[J].电化学,2011,17(2):180-185. |
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| 作者姓名： | 汪红梅 林丽清 翁少煌 王丽满 刘银环 林新华 |
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| 作者单位： | 福建医科大学药学院，福建 福州 350004 |
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| 基金项目： | 国家自然科学基金项目(20675015); 国家863计划(No.2008AA02Z433); 福建省自然科学基金(No.2010J06011,2010J01032); 福建省教育厅(No.JA10155); 福建省高校产学研科技重点项目(2010Y4003)资助 |
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| 摘 要： | 基于慢性粒细胞白血病中BCR/ABL融合基因的碱基序列，设计了一种新型发夹结构锁核酸(locked nucleic acids, LNA)探针，把LNA探针通过Au-s键固定在金电极表面构建了特异的生物传感器.LNA探针与目标链DNA杂交，以自行合成的苯甲酸二聚铜配合物(Cu2(C7H5O2)4(C2H6O)2], 简称Cu(R)2]2+)为杂交指示剂，应用差示脉冲伏安法进行检测，表现出良好的响应信号.该新型锁核酸传感器能较好的区分完全互补链DNA、单碱基错配链DNA.对互补链DNA检测的线性范围为1.0×10-8～1.0×10-6 mol?L-1，检出限为2.0×10-9 mol?L-1.
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| 关 键 词： | 发夹锁核酸探针 生物传感器 BCR/ABL融合基因 苯甲酸二聚铜配合物 |
| 收稿时间： | 2010-12-07 |
Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene |
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| WANG Hong-mei,LIN Li-qing,WENG Shao-huang,WANG Li-man,LIU Yin-huan,LIN Xin-hua.Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene[J].Electrochemistry,2011,17(2):180-185. |
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| Authors: | WANG Hong-mei LIN Li-qing WENG Shao-huang WANG Li-man LIU Yin-huan LIN Xin-hua |
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| Institution: | WANG Hong-mei,LIN Li-qing,WENG Shao-huang,WANG Li-man,LIU Yin-huan,LIN Xin-hua(College of Pharmacy,Fujian Medical University,Fuzhou 350004,China) |
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| Abstract: | In this article,a new type of structure hairpin locked nucleic acids(LNA) probe was designed to detect BCR/ABL fusion gene in Chronic Myelocytic Leukemia.The LNA probe was immobilized on the gold electrode(AuE) through sulfur-Au interaction to construct specific electrochemical biosensor.The electrochemical response of the sensor to hybridization of the LNA probe with the target DNA was studied using Cu(R)2]2+ as an electrochemical indicator.The optimal condition was discussed.The experimental results indi... |
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| Keywords: | hairpin LNA probe electrochemical biosensor BCR/ABL fusion gene [Cu2(C7H5O2)4(C2H6O)2] |
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