| 石墨炉原子吸收光谱法间接测量尿样中氨基多糖含量 |
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| 引用本文： | 王桂珍,王军.石墨炉原子吸收光谱法间接测量尿样中氨基多糖含量[J].光谱实验室,1994,11(1):30-33. |
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| 作者姓名： | 王桂珍 王军 |
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| 作者单位： | 同济医科大学公共卫生学院环境卫生学教研室 |
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| 摘 要： | 本文采用阿利新蓝（ＡｌｃｉａｎＢｌｕｅ）与尿样中氨基多糖（以下称ＧＡＧ）在ｐＨ＝５．８的醋酸钠溶液中，发生沉淀反应生成ＧＡＧ铜复合物。离心分离沉淀物，以石墨炉原子吸收光谱测定沉淀物中铜含量，间接计算ＧＡＧ含量。本文对尿样加入染液浓度，沉淀反应平衡时间；沉淀物的洗涤剂、洗涤次数等进行了探讨，确定了最佳实验条件，实测了６０例健康人尿中ＧＡＧ含量，与文献报导值一致。
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| 关 键 词： | 粘多糖 阿利新蓝 间接法 |
Indirect Measurement of Content of Urine GAG with Graphite Furnace AAS |
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| Wang Guizheng, Wang Jun, Li Heng.Indirect Measurement of Content of Urine GAG with Graphite Furnace AAS[J].Chinese Journal of Spectroscopy Laboratory,1994,11(1):30-33. |
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| Authors: | Wang Guizheng Wang Jun Li Heng |
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| Abstract: | The deposit of GAG-Cu compound was obtained from Alcian Blue and urine GAG. In sodium acetate solution of magnesium chloride(pH= 5. 8). The deposit was separated by centrifugalization. Cu-absorption was measured with Graphit furnace AAS after washing and nitration, and the content of urine GAG was calculated indirectly. In this research, Alcian concentration in Urine, deposition balance time, deposit washing substance and washing times, et al. experimental conditions were studied in detail. 60 normal human urine GAG content was measured in the best experimental condition and the result was satisfied. |
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| Keywords: | GAG Alcian Blue indirect method |
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