Performance evaluation of a high-field orbitrap mass analyzer |
| |
Authors: | Alexander Makarov Eduard Denisov Oliver Lange |
| |
Institution: | aThermo Fisher Scientific, Bremen, Germany |
| |
Abstract: | A new design of the Orbitrap™ mass analyzer is presented. Higher frequencies of ion oscillations and hence higher resolving power over fixed acquisition
time are achieved by decreasing the gap between the inner and outer Orbitrap electrodes, thus providing higher field strength
for a given voltage. Experimental results confirm maximum FWHM resolving power in excess of 350,000 at m/z 524 and 600,000 at m/z 195, isotopic resolution of proteins above 40 kDa, and a single-shot dynamic range of 25,000. It was also found that mass
shifts in the new design depend very little on space charge inside the analyzer. This performance was achieved using higher
voltages and by careful balancing of construction tolerances and operation parameters, which appeared to vary in narrower
ranges of tuning than for a standard Orbitrap analyzer. |
| |
Keywords: | |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |
|