UV-densitometric method for the determination of estrogens and androgens in pharmaceutical preparations

Summary UV Densitometric Method for the Determination of Estrogens and Androgens in Pharmaceutical Preparations A convenient method for the determination of estradiol benzoate/testosterone propionate and of ethynylestradiol/methyltestosterone mixtures from ampoules and lingualets, is described. The procedure is simple since the method involves direct reading from the thin-layer plate, avoiding thus the elution of the spot.After TL Chromatographie separation of active substances, densitometric measurements were performed with a HPTL scanner at 254 nm, whereby good and precise results were obtained. The standard deviation was found to range from 0.022 to 0.183, whereas the relative standard deviation ranged from 0.93 to 2.7%.The results obtained show that the method is applicable to routine analysis.