Template-directed DNA photoligation via alpha-5-cyanovinyldeoxyuridine |
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| Authors: | Ogino Masayuki Yoshimura Yoshinaga Nakazawa Akio Saito Isao Fujimoto Kenzo |
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| Institution: | The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa. |
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| Abstract: | reaction: see text] We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha(C)U). An efficient photoligation was produced by photoirradiation of an ODN containing alpha(C)U at the 3' end with an ODN containing thymine at the 5' end in the presence of a template ODN. This photoligation method is a new and efficient way to synthesize branched ODNs. |
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