尿液中麻黄碱与可待因的扫集胶束电动色谱法快速测定

采用扫集胶束毛细管电泳,建立了快速测定尿液中麻黄碱和可待因含量的方法,并通过日内、日间实验对方法的稳定性进行考察。讨论了pH值、十二烷基硫酸钠（SDS）浓度、分离电压、进样时间等因素的影响。建立了扫集胶束电动色谱的最佳实验条件,其中pH 2.2缓冲体系含80 mmol/L SDS,20 mmol/LNaH2PO4,18%（体积分数）乙腈,分离电压-20 kV,测量波长200 nm。在优化条件下,麻黄碱和可待因均在7 min内出峰,方法检出限（mg/L）、线性范围（mg/L）、相关系数分别为麻黄碱0.173、0.693-11.1、0.9993,可待因0.333、1.33-16.0、0.9993,应用于实际样品测定,回收率为94%-108%,RSD不大于3.5%。峰面积日内RSD不大于6.3%（n=5）,日间RSD不大于9.3%（n=5）。

Rapid Determination of Ephedrine and Codeine in Human Urine by Sweeping Micellar Electrokinetic Chromatography

The sweeping micellar electrokinetic chromatography(Sweeping-MEKC)method was developed for the determination of ephedrine(Ep) and codeine(Co) in human urine sample.Stability of this method was evaluated by intra-day and inter-day experiments.The effects of pH,SDS concentration,separation voltage and injection time on the separation efficiency were discussed.The optimal conditions were using pH 2.2 20 mmol/L NaH2PO4 as buffer containing 80 mmol/L SDS,and 18% ACN(by volume),applying separation voltage of-20 k...