Direct chiral separation of caderofloxacin enantiomers by HPLC using a glycoprotein column |
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| Authors: | W Zheng X Xu E Wang |
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| Institution: | (1) State Key laboratory of Organometailic Chemistry, Laboratory of Analytical Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, China;(2) Institute of Medicine and Chemical Engineering, Chinese Pharmaceutical University, Nanjing, 210009, China |
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| Abstract: | The enantiomers of caderoflxacin (CS-940), the new antibacterial fluoroquinolone compound, were separated on the commercially available α-acid glycoprotein-coated chiral stationary phase (Chiral-AGP) using the mobile phase of IPA: 0.15 M NaH2PO4 + Et3N (pH 7.9) = 3: 97 at 0.8 mL/min with UV detection at 282 nm. The chromatographic behavior of caderofloxacin enantiomers was investigated by varying the mobile phase conditions. The chiral assay method was validated and used to determination of (R)-Caderofloxacin in (S)-Caderofloxacin raw material samples. |
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