| 醋酸纤维素膜固定化脲酶的研究 |
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| 引用本文： | 王杰,宋锡瑾,伍明,任仲皎,袁斌,雷灼霖.醋酸纤维素膜固定化脲酶的研究[J].高等学校化学学报,1998,19(7):1104-1106. |
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| 作者姓名： | 王杰 宋锡瑾 伍明 任仲皎 袁斌 雷灼霖 |
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| 作者单位： | 1. 杭州大学化学系, 杭州, 310028;
2. 浙江大学化工系, 杭州, 310027;
3. 中南民族学院化学系, 武汉, 430074 |
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| 摘 要： | 酶固定膜反应器兼具有反应和分离两种功能,是酶工程领域中较活跃的研究课题.随着酶固定化技术和水平的提高,各种固定化酶生物反应器不断涌现,其中以采用固定化脲酶技术制作的人工肾最为成功.
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| 关 键 词： | 醋酸纤维素膜 固定化 脲酶 |
| 收稿时间： | 1997-05-21 |
Studies on Immobilization of Urease on Cellulose Acetate Membrane |
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| WANG Jie,SONG Xi-Jin,WU Ming,REN Zhong-Jiao,YUAN Bin,LEI Zhuo-Lin.Studies on Immobilization of Urease on Cellulose Acetate Membrane[J].Chemical Research In Chinese Universities,1998,19(7):1104-1106. |
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| Authors: | WANG Jie SONG Xi-Jin WU Ming REN Zhong-Jiao YUAN Bin LEI Zhuo-Lin |
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| Institution: | 1. Department of Chemistry, Hangzhou University, Hangzhou, 310028;
2. Chemical Engineering Department, Zhejiang University, Hangzhou, 310027;
3. Department of Chemistry, The South Central College for Na iona lities , Wuhan, 430074 |
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| Abstract: | Urease was immobilized on cellulose acetate membrane by entrapment. The activity yield and stability of urease immobilized membrane were investigated in catalyzing hydrolysis of urea. The experimental results showed that the activity of urease membrane was related to water content of casting membrane solution. After the enzymatic membrane was used repeatedly for 10 times, the residual activity of urease on the membrane retained 60% of its original. After storage at 4 ℃ for 3 months almost no decrease of enzymatic activity was observed. The optimal pHand temperature of the hydrolysis reaction were also studied. The results showed that this urease immobilized membrane can be used under a wide operational condition. |
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| Keywords: | Cellulose acetate membrane Immobilization Urease |
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