Amperometric biosensor for rapid measurement of 3-hydroxybutyrate in undiluted whole blood and plasma |
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| Authors: | Calum J McNeil Julia A Spoors Jonathan M Cooper K George MM Alberti William H Mullen |
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| Institution: | Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH Great Britain;Department of Medicine, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH Great Britain;Cambridge Life Sciences plc, Angel Drove, Ely CB7 4DT Great Britain |
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| Abstract: | A simple, rapid (< 30 s) electrochemical method for the determination of 3-hydroxybutyrate in whole blood or plasma is described, which uses NAD+-dependent d-3-hydroxybutyrate dehydrogenase immobilized at novel platinized carbon electrodes. The steady-state oxidation current produced by enzymatically generated NADH is measured at + 150 mV vs. Ag/AgCl. Enzyme electrodes produced by direct adsorption were stable for at least 3 months. Undiluted whole blood measurement with the sensor was compared with routine spectrophotometric analysis of plasma and perchloric acid extracts of whole blood. |
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| Keywords: | Hydroxybutyrate Blood Plasma Biosensor Enzyme electrodes |
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