Paper electrophoresis with surface-enhanced fluorimetric detection |
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Authors: | YF Cheng T Vo-Dinh |
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Institution: | Advanced Monitoring Development Group, Health and Safety Research Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101 U.S.A. |
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Abstract: | Amorphous fumed silica was used as a fluorescence sensitizer coated on a filter-paper substrate for use in paper electrophoresis. The usefulness of the separation method was illustrated for a mixture of two polynuclear aromatic compounds of biological interest: benzoa]pyrene (BaP) and benzoa]pyrene-r-7,t-8,9,10-tetrahydrotetrol, a hydrolysis product of BaP—DNA adduct. These two compounds were well separated and an enhancement of the fluorescence intensity was also observed when the sensitizer was applied to filter-paper prior to electrophoretic separation. |
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