Rapid determination of microorganisms using a flow-injection system |
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Authors: | Thomas Ding Rolf D Schmid |
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Institution: | GBF - Gesellschaft für Biotechnologische Forschung, Department of Enzyme Technology, Mascheroder Weg 1, D-3300 Braunschweig F.R.G. |
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Abstract: | An automated method for the rapid determination of microorganisms using a flow-injection system is presented. Electrochemical measurement of a mediator reduced by microbial metabolism allowed the determination of fungi and bacteria in a few minutes. The lowest detection limit was 5 × 106 colony-forming units (cfu) ml?1 for Escherichia coli. Correlation between the flow-injection method and standard microbiological methods was excellent (r = 0.997, n = 4 for Beauveria bassiana; r = 0.997, n = 7 for E. coli). The flow-injection system was applied to the on-line control of an E. coli cultivation. |
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