Determination of l-alanine in a flow-injection system with an immobilized enzyme reactor |
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| Authors: | Nobutoshi Kiba Hirohisa Tagami Motohisa Furusawa |
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| Institution: | Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu 400 Japan |
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| Abstract: | A flow-injection system for the determination of l-alanine is described. Alanine dehydrogenase is immobilized on poly(vinyl alcohol) beads and used in a packed-bed enzyme reactor. The system responds linearly to injected samples (50 μl) in the concentration range 0.5–500 μM. The maximum throughput was 40 samples per hour. The immobilized enzyme reactor was stable for at least 6 weeks. Its usefulness for assay of l-alanine in serum and beverages is described. |
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| Keywords: | Alanine Enzyme reactor Serum Beverages |
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