Micro-enzyme field effect transistor sensor using direct covalent bonding of urease |
| |
| Authors: | F. Gardies N. Jaffrezic-Renault C. Martelet H. Perrot J.-M. Valleton S. Alegret |
| |
| Affiliation: | Laboratoire de Physicochimie des Interfaces, URA CNRS 404, Ecole Centrale de Lyon, BP 163, F-69131 Ecully Cédex France;URA CNRS 500, Université de Rouen, F-76134 Mont Saint Aignan France;Universidad Autonoma de Barcelona, Facultad de Ciencias, Departamento de Quimica, 08 193 Bellaterra Spain |
| |
| Abstract: | Enzyme field effect transistors (ENFETs), which have been previously proposed for the detection of urea, consist of a pH ion-sensitive FET with urease enzyme immobilized in a polymeric membrane. A new means of preparing the enzymatic sensing layer is proposed in which urease is directly covalently bonded onto the silica insulator. The sensitivity, lifetime and response time of the ENFETs obtained are fairly good. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
