Preparation of small-scale samples from insect for chromatography

Techniques are available for the gas chromatography of very small samples of insect pheromones and defensive secretions by which the diluting and contaminating effects of solvents are avoided. Samples obtained in many ways can be sealed in fine glass capillaries, heated and crushed on-column to give quantitative high-resolution chromatograms. Reactions can be carried out in a micro-reactor and the reaction products obtained in a very small volume (5 μl) for chromatography without evaporation.