Spectrophotometric determination of hydrogen peroxide with leuco Patent Blue Violet

Hydrogen peroxide (1 × 10^?7?10^?5 mol dm^?3) was determined in aqueous solution using leuco Patent Blue Violet and peroxidase. The reagent can also be applied in the same concentration range to the ethyl acetate extraction method described previously. This allows hydrogen peroxide to be determined in the presence of a variety of species which interfere in the direct method. A reagent consisting of leuco Patent Blue Violet, peroxidase, glucose oxidase and mutarotase was also applied to the determination of glucose.