Department of Environmental Engineering, Saitama Institute of Technology, 1690, Fusaiji, Okabe, Saitama 369-02 Japan;Research Centre, Osaka Gas Co., Ltd., 6-19-9, Torishima, Konohana-ku, Osaka 554 Japan
Abstract:
A novel flow-coulometric detector integrating an immobilized uricase reactor and an electrolytic cell was fabricated and used for the determination of uric acid in human urine. The procedure is based on the measurement of the total charge with and without passing the sample through an enzyme reactor which allows the complete conversion of the electro-active uric acid in electro-inactive products. The amount of uric acid is linearly related to the difference between the two total charges. The current efficiencies for 1 × 10?4-1 × 10?3 M uric acid were found to be nearly 100% (r.s.d. < 1%).