Phosphate-sensitive enzyme electrode: a potential sensor for environment control |
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Authors: | E.M. d Urso,P.R. Coulet |
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Affiliation: | Laboratoire de Génie Enzymatique, UMR 106 CNRS, Université Lyon 1, 43 Bd. du 11 Novembre 1918, 69622 Villeurbanne Cédex France |
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Abstract: | A highly sensitive enzyme electrode was designed for the assay of phosphate ions. For this purpose, a bienzyme membrane with co-immobilized nucleoside phosphorylase and xanthine oxidase was used with a platinum amperometric electrode for the detection of enzymatically generated hydrogen peroxide. A detection limit of 10?7 M was obtained and phosphate assays could be easily performed in the range 0.1–10 μM, which is of interest in the control of water pollution. |
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Keywords: | Enzyme electrode Phosphate Waters |
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