Application of a coated-wire electrode to an acetylcholine sensor |
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| Authors: | H Taguchi N Ishihara K Okumura Y Shimabayashi |
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| Institution: | Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University, Tsu, Mie 514 Japan |
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| Abstract: | An acetylcholine sensor was constructed with acetylcholinesterase which was immobilized on a hydrogen ion-selective coated-wire electrode and fundamental properties of this sensor were investigated. Acetylcholine could be determined in the range 0.1–10 rum with response times of 3–10 min. The effects of pH and concentration of buffer solution on the determination and fluctuations in the data obtained with this sensor were also investigated. Possibilities for the practical use of this acetylcholine micro-sensor are suggested. |
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| Keywords: | Acetylcholine Coated-wire electrode Biosensors Enzyme electrodes |
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