Determination of hydrogen peroxide by photoinduced fluorogenic reactions |
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Authors: | Zhang Genfa Purnendu K Dasgupta William S Edgemond John N Marx |
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Institution: | Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061 U.S.A. |
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Abstract: | 4-Hydroxyproline, 8-hydroxyquinoline, 4-hydroxyquinoline-2-carboxylic acid and 4-hydroxyphenylacetic acid (HPA) react with H2O2 when irradiated with UV light to yield fluorescent products. Sub-micromolar detection limits of H2O2 are possible with HPA. The fluorescent product is the same as that formed in the peroxidase enzyme-mediated H2O2 oxidation of HPA. Several compounds that participate in the photomediated reaction do not react in the enzyme-mediated system. A mechanism for the photomediated reaction involving an aryloxy radical derived from the substrate is suggested. Although the limits of detection for H2O2 do not equal the best achievable with the enzyme-mediated systems, the simplicity of a single-step reaction and an ability to “photodevelop” the product offer a range of novel analytical possibilities. |
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Keywords: | Fluorimetry Flow system Hydrogen peroxide Photoinduced fluorogenic reactions |
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