Chemiluminescence determination of vitamin B12 by a flow-injection method |
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Authors: | Y.K. Zhou H. Li Y. Liu G.Y. Liang |
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Affiliation: | Institute of Environmental Medicine, Tongji Medical University, Wuhan, Hubei 430030 China;School of Pharmacy, Tongji Medical University, Wuhan, Hubei 430030 China |
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Abstract: | Chemiluminescence analysis with flow injection was used for the determination of vitamin B12 by means of the luminol-hydrogen peroxide system. The linear range is three orders of magnitude and the detection limit is 1 ng for 50-μ1 samples injected. The results were compared with those obtained by a spectrophotometric method. |
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Keywords: | Chemiluminescence Flow system |
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