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Chemiluminescence determination of vitamin B12 by a flow-injection method
Authors:Y.K. Zhou  H. Li  Y. Liu  G.Y. Liang
Affiliation:Institute of Environmental Medicine, Tongji Medical University, Wuhan, Hubei 430030 China;School of Pharmacy, Tongji Medical University, Wuhan, Hubei 430030 China
Abstract:Chemiluminescence analysis with flow injection was used for the determination of vitamin B12 by means of the luminol-hydrogen peroxide system. The linear range is three orders of magnitude and the detection limit is 1 ng for 50-μ1 samples injected. The results were compared with those obtained by a spectrophotometric method.
Keywords:Chemiluminescence  Flow system
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