Grape tissue-based electrochemical sensor for the determination of hydrogen peroxide |
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Authors: | Daya Wijesuriya Meng Shan Lin Garry A. Rechnitz |
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Affiliation: | Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii at Manoa, 2545 The Mall, Honolulu, Hawaii 96822 U.S.A. |
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Abstract: | The use of grape tissue as a source of catalase for the determination of hydrogen peroxide is reported. A slice of grape tissue attached to the membrane of a Clark-type oxgen sensor was used to monitor the oxidation of hydrogen peroxide by catalase. At the steady state, the sensor responds linearly to hydrogen peroxide in the concentration range 1 × 10?5–5 × 10?4 M. The response time (T90) was of the order of 1 min for this sensor. No interference was observed from ethanol, amino acids, glucose and lactic acid. The long-term stability of the grape tissue sensor was much better than previously reported immobilized enzyme and liver tissue-based hydrogen peroxide sensors. |
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Keywords: | Hydrogen peroxide |
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