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Sensor for free fatty acids based on acyl coenzyme-a synthetase and acyl coenzyme-a oxidase
Authors:Koji Sode  Eiichi Tamiya  Isao Karube  Yuko Kameda
Affiliation:Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153 Japan;Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 227 Japan;Research Section 1, Food, Oil and Fat Laboratories, Asahi Electro-Chemical Company, 8-4-1, Higashiogu, Arakawa-ku, Tokyo 116 Japan
Abstract:The measurement is based on monitoring dissolved oxgyen consumed by the two sequential reactions catalyzed by the enzymes immobilized in photo-cross-linkable poly (vinyl alcohol) resin (PVA-SbQ). A linear correlation was observed between current decrease and 0.3–2.6 mM oleic or palmitic acid. The sensor was stable in use for three days and could be used to monitor the freshness of oily food.
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