Simultaneous determination of glucose and sucrose by a glucose-sensing enzyme electrode combined with an invertase-attached cell |
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Authors: | Fumio Mizutani Michihiko Asai |
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Affiliation: | Research Institute for Polymers and Textiles, Higashi 1-1-4, Tsukuba, Ibaraki 305 Japan |
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Abstract: | Glucose and sucrose are simultaneously determined by using a glucose-sensing enzyme electrode combined with a cell that contains immobilized invertase. The electrode current changes linearly with time for several minutes from ca. 1 min after the addition of a glucose-sucrose mixture. The concentration of sucrose (60 μM-6 mM) is determined from the rate of current change in the linear region, and that of glucose (5 μM-1 mM) is determined by extrapolating the straight current-time line to t=0.45 min and by measuring the intercept on the vertical (current) axis at t=0.45 min. The relative standard deviations are 1.8% for glucose and 3.7% for sucrose (n=10). More than 20 food samples can be analysed in 1 h. |
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Keywords: | Enzyme electrode Glucose Sucrose |
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