Bienzyme amperometric lactate-specific electrode |
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| Authors: | H. Durliat C. Causserand M. Comtat |
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| Affiliation: | Laboratoire de Génie Chimique, URA CNRS 192, Laboratoire de Chimie Physique et Electrochimie, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cédex France |
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| Abstract: | The electrode, based on a lactate dehydrogenase and a diaphorase, permits the assay of l-lactate in the concentration range 0.2–8 mM with a response time of about 40 s. Both the enzymes are commercially available. The amperometric detection of hexacyanoferrate(II) at a platinum electrode is done at 0.3 V (vs. SCE) instead of 0.8 V as in the detection of NADH, improving the selectivity of the sensor. |
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