Response characteristics of the glucose-sensitive field-effect transistor. Computer simulation of the effect of gluconolactonase coimmobilization in a glucose oxidase membrane |
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Authors: | Y Hanazato S Shiono M Maeda |
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Institution: | Central Research Laboratory, Mitsubishi Electric Corporation, 1-1, Tsukaguchi-Honmachi-8-Chome, Amagasaki, Hyogo 661 Japan |
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Abstract: | The bienzyme system consisting of glucose oxidase and gluconolactonase was investigated using a conventional diffusion-kinetics model for an enzyme-modified field-effect transistor (FET) to clarify the effect of gluconolactonase coimmobilization in a glucose oxidase membrane on the steady-state response amplitude of a glucose sensor based on a FET. The model includes spontaneous and enzymatic hydrolysis reactions of d-glucono-δ-lactone and it elucidated the following experimental results: a glucose sensor with a membrane (about 1 μm in thickness) coimmobilizing these enzymes showed a sufficient response amplitude, whereas without coimmobilization of gluconolactonase no detectable response was observed up to 3 mM glucose; and the response amplitude depended strongly on the amount of lactonase in the membrane. The model also predicted an optimum enzyme ratio for coimmobilization in a membrane. |
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