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Study of the mimetic peroxidase-catalysed chemiluminescence reaction
Authors:Hong-Bin He
Institution:Department of Chemistry, Peking University, Beijing China
Abstract:The chemiluminescence behaviour of the reaction in which the Mn-TPPS4 complex the mimetic enzyme of peroxidase manganese tetrakis(sulphophenyl)porphine] acts as a catalyst for the oxidation of luminol by hydrogen peroxide was studied. The reaction product luminesces at 427 nm. Trace amounts of hydrogen peroxide and glucose can be determined with detection limits of 5.5 × 10?9 and 2.7 × 10?9 M, respectively. The characteristics of Mn-TPPS4 were compared with those of horseradish peroxidase.
Keywords:Enzyme reactions  Glucose  Serum
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