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Effects of carbodiimides as coupling agents on the stability of immobilized aldolases |
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| Authors: | Magdolna Ábrahám Zsolt Pénzes Béla Szajáni |
| Affiliation: | 1. Department of Biochemistry, Attila József University, PO Box 533, H-6721, Szeged, Hungary 2. Institute of Biophysics, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary 3. Reanal Factory of Laboratory Chemicals, PO Box 54, Budapest 70, Hungary |
| Abstract: | Pig muscle aldolase was covalently attached to a polyacrylamide support possessing carboxylic functional groups activated by various water-soluble disubstituted carbodiimides. The stability and the degree of microheterogeneity of the immobilized aldolases were shown to depend on the structure of the carbodiimide used as coupling agent. |
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