PHYCOBILISOMES AND ISOLATED PHYCOBILIPROTEINS. EFFECT OF GLUTARDIALDEHYDE AND BENZOQUINONE ON FLUORESCENCE |
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| Authors: | H.-P. Kö st,,K. Rath,,G. Wanner, H. Scheer |
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| Affiliation: | Botanisches Institut der Universität, D-8000 München 19, W. Germany |
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| Abstract: | Abstract— The fluorescence of the biliproteins C-phycocyanin from Spirulina platensis , B-phycoerythrin from Porphyridium cruentum and of isolated whole P. cruentum phycobilisomes is quenched in the presence of glutardialdehyde (GA) or benzoquinone (BQ). The kinetics of fluorescence decrease thus induced is biphasic. If GA is used as a quencher, the fluorescence can be recovered at 77 K. Contrary to the GA-effect, only a minor recovery takes place with BQ at 77 K, thus demonstrating a different mechanism of action of GA and BQ on biliproteins. |
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