| 人血多形核白细胞呼吸爆发中活性氧与活性氧模拟酶的关系研究 |
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| 引用本文： | 冯清,李世新.人血多形核白细胞呼吸爆发中活性氧与活性氧模拟酶的关系研究[J].分析化学,2000,28(11):1395-1397. |
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| 作者姓名： | 冯清 李世新 |
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| 作者单位： | [1]同济医科大学化学教研室,武汉 [2]同济医科大学附属同济医院检验科,武汉 |
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| 基金项目： | 国家自然科学基金!资助课题 (No .39370 794 ) |
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| 摘 要： | 用多形核白细胞－鲁米诺化学发光法测定具有超氧化物歧化酶（ＳＯＤ）和过氧化氢酶（ＣＡＴ）双功能模拟酶－金属卟啉（四磺酸基苯基铁卟啉、四磺酸基苯基铜卟啉、四磺酸基苯基锰卟啉、四磺酸基苯基钴卟啉）对呼吸爆发所产生的活性氧的影响，结果表明，模拟酶对爆发反应中细胞发光产生抑制，即对活性氧有清除作用；且同一物质随着浓度由１０＾－７－１０＾－５ｍｏｌ／Ｌ，上升而增强。
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| 关 键 词： | 多形核白细胞 模拟酶 呼吸爆发 活性氧 化学发光 |
A Study on the Measurement of Active Oxygen During the Respiratory Burst in Polymorphonuclear Leucoyte and Relationship of Mimetic Enzyme as Potential Anti-active Oxygen |
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| Abstract: | The active oxygen released by PMN during the respiratory burst was measured with PNM luminol enhanced chemiluminescence, the result showed that the metal porphyrins (tetraphenylporphyrinesulfonatoiron, tetraphenylporphyrinesulfonatocopper, tetraphenylporphyrinesulfonatomanganese, tetraphenylporphyrinesulfonatocobalt) used as dual function mimicry of superoxide dismutase (SOD) and catalase (CAT) inhibit chemiluminescence, scavenge the active oxygen. The percentage of scavenging active (O - 2, H 2O 2) increased with increasing concentration of the imitating compounds from 10 -7 mol/L to 10 -5 mol/L. |
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| Keywords: | Polymorphonuclear leucocyte mimetic enzyme respiratory burst active oxygen |
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