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Fermentation monitoring using multisensor systems: feasibility study of the electronic tongue
Authors:Kim?Esbensen  Dmitry?Kirsanov  Andrey?Legin  Email author" target="_blank">Alisa?RudnitskayaEmail author  John?Mortensen  Joan?Pedersen  Lene?Vognsen  Sergey?Makarychev-Mikhailov  Yuri?Vlasov
Institution:(1) Center for Biotechnology and Bioenergy (IB2) & Applied Chemometrics, Analytical Chemistry and Sampling Research Group (ACACSRG), Aalborg University, Esbjerg, Denmark;(2) Laboratory of Chemical Sensors, Chemistry Department, St. Petersburg University, St. Petersburg, Russia;(3) Roskilde University Centre, Roskilde, Denmark;(4) Biotechnological Institute, Kolding, Denmark;(5) Arla Food Innovation, Denmark
Abstract:The electronic tongue based on an array of 30 non-specific potentiometric chemical sensors has been applied to qualitative and quantitative monitoring of a batch fermentation process of starting culture for light cheese production. Process control charts were built by using PLS regression and data from fermentations run under ldquonormalrdquo operating conditions. Control charts allow discrimination of samples from fermentation batches run under ldquoabnormalrdquo operating conditions from ldquonormalrdquo ones at as early as 30–50% of fully evolved fermentations. The capability of the electronic tongue to quantify concentrations of important organic acids (citric, lactic and orotic) in the present type of fermentation media was demonstrated. Average prediction errors were assessed in the range 5–13% based on test set validation. Correlation between peptide profiles determined using HPLC and the electronic tongue output was also established. The electronic tongue was demonstrated to be a promising tool for fermentation process monitoring and quantitative analysis of growth media.http://www.electronictongue.com
Keywords:Electronic tongue  Fermentation monitoring  Process control  Determination of organic acids
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