毛细管电泳安培测定鼠单个交感神经细胞中儿茶酚胺类神经递质

用自组装的毛细管电泳微电极安培检测系统，分析了鼠的单个交感神经细胞。大白鼠经麻醉、微解剖及酶解分离出新鲜的单个交感神经细胞。采用全细胞进样，用异丙肾上腺素 作内标。测定了儿茶酚胺类神经递质去甲肾上腺素和肾上腺素。检测限分别达 ５７ ａｍｏｌ和 ５９ ａｍｏｌ。八个活细胞分析结果，每个细胞平均含去甲肾上腺素 ４５５ ａｍｏｌ及肾上腺素 ６５ ａｍｏｌ。

Determination of Catecholamines in Single Sympathetic Nerve Cell of Rat by Capillary Electrophoresis With Amperometric Detection

This paper reports the use of a home-made capillary electrophoresis (CE) with amperometric detection system to the analysis of neurotransmitters in a single sympathetic nerve cell (diameter 15-25 um, volume 2-5 pL) of rat. After enzymolysis the cells were isolated. Sampling of the fresh whole cell was performed through electromigration by directly drawing a cell into the tip of a capillary and lysing the cell inside the capilary as observed under the microscope. The catecholamines in a single cell were separated by CE at the sepa- ration potential of 15 kV. Electrochemical detection was performed at 0. 7 V vs. SCE. Isoproterenol was selected to be the internal standard for the determination of norepinephrine (NE) and epinephrine (E) in a single sympathetic nerve cell. The detection limits for NE and E are 57 amol and 59 amol, respectively. The average analytical results of NE and E in eight single sympathetic nerve cells of rats are 445 amol and 65 amol/per cell, respectively.