Enzymatic hydrolysis of cellulose hydrates |
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Authors: | Kayoko Kobayashi Satoshi Kimura Ung-Jin Kim Ken Tokuyasu Masahisa Wada |
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Institution: | (1) Department of Biomaterials Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan;(2) Department of Plant and Environmental New Resources, College of Life Sciences, Kyung Hee University, 1, Seocheon-dong, Giheung-ku, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea;(3) Bioenergy Research Center, College of Life Sciences, Kyung Hee University, 1, Seocheon-dong, Giheung-ku, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea;(4) Food Resource Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan; |
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Abstract: | We prepared two cellulose hydrates, Na-cellulose IV and cellulose II hydrate, along with their respective anhydrous forms,
cellulose II and II′, from microcrystalline cellulose. X-ray diffractometry analysis showed that the structure of the hydrophobic
stacking sheet was conserved in the samples, but the distance between the sheets was in the order: cellulose II hydrate > Na-cellulose
IV > cellulose II and II′. The hydrates exhibited an expanded structure compared with the anhydrous form from the incorporation
of hydrate water, and cellulose II hydrate contained more hydrate water than Na-cellulose IV. Enzymatic hydrolysis of the
samples was carried out at 37 °C using solutions comprising a mixture of cellulase and β-glucosidase. The hydrates were hydrolyzed
more efficiently than the anhydrous forms, and cellulose II hydrate showed a more efficient hydrolysis than Na-cellulose IV.
This result also agrees well with the enzymatic adsorption properties of each sample, where the samples that adsorbed the
greater amount of enzyme showed a higher degradability. The results obtained in this study provide useful knowledge on controlling
the biodegradability of cellulose by converting its structure. |
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