Construction and Expression of Human Survivin and Preparation of Its Polyclonal Antibody |
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Authors: | Hai-hong ZHANG Xi-zhen ZHANG Dong-hai ZHAO He-liang SHI Yong-hui YU Yong-ge WU Xiang-hui YU Wei KONG |
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Institution: | Vaccine Research Center, College of Life Science, Jilin University, Changchun 130012, P R. China |
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Abstract: | Survivin,a novel member of inhibitor ofapoptosis(IAP) protein family,is aberrantly expressed in cancer but undetectable in normal,differentiated adult tissues.The cancer-specific expression of survivin,coupled with its importance in inhibiting cell death and in regulating cell division makes it a useful diagnostic marker of cancer and a potential target for cancer treatment.Survivin cDNA amplified from the total RNA of 293 cells through RT-PCR was cloned into prokaryotic expression vector pRSET-B.The recombinant plasmid pRSET-B-Surv was expressed in E.coli BL21,and the relative molecule mass(Mr) of expressed fusion protein was approximately 21000.The recombinantprotein was purified through Ni2~ affinity chromatography column and characterized by SDS-PAGE and Western blot.The purified recombinant protein was then injected into rabbits,and antisurvivin polyclonal antibody with a high titer was obtained. |
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Keywords: | Clone Survivin Expression Polyclonal antibody |
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