芪参补气药茶清除线粒体活性氧研究

以黄芪和党参为原料,根据中医学原理组方制成芪参补气药茶(ACT),探索ACT清除线粒体活性氧作用及促进健康的机制。分别用AlCl₃比色法和硫酸苯酚法测定ACT的功能因子总黄酮、总糖及总多糖含量。以还原型辅酶Ⅰ/吩嗪硫酸甲酯(NADH/PMS)为超氧阴离子(O₂·^－)生成系统,过氧化氢(H₂O₂)/Fe²⁺体系为羟自由基(·OH)生成系统,分别用氮蓝四唑(NBT)还原法和Fenton反应显色法测定ACT清除O₂·^－及·OH的能力;用Na₂S₂O₃滴定法测定ACT清除H₂O₂的能力。ACT总糖质量分数为(26±1.3) mg·mL^-1,总多糖质量分数为(12.5±0.8) mg·mL^-1,总黄酮质量分数为(121±8.5) μg·mL^-1。ACT可一定程度上清除O₂·^－、·OH和H₂O₂。ACT具有温和的抗氧化作用,从而维持机体氧化与抗氧化平衡,这是ACT促进健康的潜在机制。

Mitochondrial Reactive Oxygen Species Scavenging Investigations of Astragalus Membranaceus/Codonopsis Pilosula Qi-invigorating Herbal Tea

Astragalus membranaceus/Codonopsis pilosula Qi-invigorating herbal tea (ACT) was prepared from Astragalus membranaceus and Codonopsis pilosula according to traditional Chinese medicine principle. Mitochondrial reactive oxygen species scavenging activity of ACT and the underlying health-promoting mechanism were investigated. Total flavonoids, total carbohydrates and total polysaccharides, which are the functional factors of ACT, were determined by AlCl₃ colorimetry, and sulfuric acid and phenol method respectively. The scavenging activities of ACT on superoxide anion (O₂·^－) and hydroxyl radical (·OH), which were produced by reduced nicotinamide adenine dinucleotide (NADH)/N-Methylphenazonium methyl sulfate (PMS) and hydrogen peroxide (H₂O₂)/Fe²⁺ system respectively, were measured by NBT reduction and Fenton reaction colorimetry. The Na₂S₂O₃ titration method was used to measure the scavenging activities of ACT on H₂O₂. The contents of total carbohydrates, total polysaccharides and total flavonoids of ACT were 26±1.3 mg/mL, 12.5±0.8 mg/mL and 121±8.5 μg/mL, respectively. ACT could scavenge O₂·^－, ·OH and H₂O₂. According to the research, ACT has mild antioxidation, therefore, the balance of oxidation and antioxidant (redox balance) was maintained, and this maybe the underlying mechanism of the health benefits for ACT.