| 鹿茸寡肽的制备及其促成骨细胞的增殖作用 |
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| 引用本文： | 王华,林喆,刘强,蔡明军,徐力,张学忠. 鹿茸寡肽的制备及其促成骨细胞的增殖作用[J]. 高等学校化学学报, 2008, 29(9): 1791-1796 |
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| 作者姓名： | 王华 林喆 刘强 蔡明军 徐力 张学忠 |
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| 作者单位： | 吉林大学分子酶学工程教育部重点实验室,长春,130021;吉林大学中日联谊医院,长春,130033;长春中医药大学中医药与生物工程研究开发中心,长春,130117;吉林大学中日联谊医院,长春,130033;吉林大学分子酶学工程教育部重点实验室,长春,130021 |
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| 基金项目： | 国家中医药管理局科研项目，吉林省科技厅科研项目 |
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| 摘 要： | 从梅花鹿鹿茸中提取了天然鹿茸总多肽(VATP). 在进一步分离纯化的过程中, 筛选出具有促进成骨细胞增殖的高活性肽组分(VAP-B), 并通过制备型HPLC对其纯化, 得到了分子量分布约为200~600的小肽活性组分(VAP-B2). 细胞周期分析结果表明, 鹿茸肽VAP-B2组分促进了人成骨肉瘤细胞OS-732的周期转化, 使其细胞周期S期细胞指数明显增加, 即表现为S期DNA含量明显提高. 碱性磷酸酶(ALP)活性检测结果表明, 随着鹿茸肽VAP-B2剂量的增加, ALP的活性明显增加. 这与成骨细胞增殖分化及成熟过程中, 细胞的周期性变化和骨形成标志物碱性磷酸酶水平的明显增高相符.
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| 关 键 词： | 梅花鹿鹿茸 鹿茸肽 分离与纯化 生理活性 成骨细胞 |
| 收稿时间： | 2008-02-29 |
Preparation of Velvet Antlers Small Peptides and Stimulating Effects on Osteosarcoma Cell Proliferation |
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| WANG Hua,LIN Zhe,LIU Qiang,CAI MING-Jun,XU Li,ZHANG Xue-Zhong. Preparation of Velvet Antlers Small Peptides and Stimulating Effects on Osteosarcoma Cell Proliferation[J]. Chemical Research In Chinese Universities, 2008, 29(9): 1791-1796 |
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| Authors: | WANG Hua LIN Zhe LIU Qiang CAI MING-Jun XU Li ZHANG Xue-Zhong |
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| Affiliation: | Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China; ;China-Japan Union Hospital of Jilin University, Changchun 130033, China; ;Research and Exploitation Center of Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China |
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| Abstract: | The native total peptides(VATP)were extracted from velvet antlers of sika deer(Cerv us nippon Temminck). A high active peptide component(VAP-B)stimulating proliferation of a human osteosarcoma cells was isolated from VATP. Finally, a further purified active peptide component(VAP-B2) with molecular weight distribution from about 200—600 was obtained from VAP-B by preparative HPLC. VAP-B2 was able to enhance the average content of DNA in S phase. And also the alkali phosphatase(ALP) activity in osteosarcoma cells increased obviously with the increase of VAP-B2 dosage and treatment time. The results are consistent with cell cycle variation and ALP level as a marker of bond formation during osteocyte proliferation and differentiation, as well as maturity. The results obtained in this study provide a clue to explore a new peptide medicine from velvet antlers for therapy of osteoporosis. |
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| Keywords: | Velvet antlers of sika deer Sika deer peptides Isolation and purification Physiologic activity Osteosarcoma cells |
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