Frequency-domain fluorescence spectroscopy, a new method for the resolution of complex fluorescence emission |
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| Authors: | Joseph R Lakowicz Ignacy Gryczynski Henryk Cherek Gabor Laczko Nanda Joshi |
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| Institution: | University of Maryland at Baltimore, School of Medicine, Department of Biological Chemistry, 660 West Redwood Street, Baltimore, MD 21201, U.S.A. |
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| Abstract: | The fluorescence emission from complex chemical and biological samples can be resolved by measuring the frequency-response of the emission, which is now possible from 1 to 2000 MHz. The frequency-response allows determination of the components in a mixture, construction of time-resolved emission spectra, and measurement of the dynamic and hydrodynamic properties of biological macromolecules. The instrumentation is relatively simple, and data acquisition times can be short. At present, this method may be superior to direct measurements of time-resolved fluorescence emission. |
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