| 青霉素裂解液的高效液相色谱分析 |
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| 引用本文： | 赵静玫,都绛瑛,张铭俊.青霉素裂解液的高效液相色谱分析[J].色谱,2001,19(1):88-90. |
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| 作者姓名： | 赵静玫 都绛瑛 张铭俊 |
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| 作者单位： | 中国科学院大连化学物理研究所, |
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| 摘 要： | 利用高效液相色谱法分析测定青霉素裂解液中的主要成分 6 氨基青霉烷酸 (6 APA)、青霉素G钾和苯乙酸 ,并对副产物青霉噻唑酸进行了液相色谱的定性检测。结果表明 ,实验方法简便、快速 ,结果准确 ,可以用于 6 APA生产的质量控制。
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| 关 键 词： | 高效液相色谱法 6-氨基青霉烷酸 青霉素G钾 苯乙酸 青霉噻唑酸 青霉素酰化酶 青霉素酶 |
| 文章编号： | 1000-8713(2001)01-0088-03 |
| 修稿时间： | 2000年3月15日 |
Determination of Degraded
Products of Penicillin by High Performance Liquid Chromatography |
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| ZHAO Jing mei,\ DU Jiang ying,\ ZHANG Ming jun.Determination of Degraded
Products of Penicillin by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2001,19(1):88-90. |
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| Authors: | ZHAO Jing mei \ DU Jiang ying \ ZHANG Ming jun |
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| Institution: | Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. zhaomei@dicp.ac.cn |
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| Abstract: | A method for the determination of main composition in degraded products of penicillin, 6-aminopenicillanic acid (6-APA), penicillin G kalium (PGK), phenylacetic acid (PAA), and the by-product benzylpenicilloic acid (BPA) by HPLC was studied. Separation conditions were as follows: Spherisorb C18 column, 250 mm x 4.6 mm i.d., 10 micrograms; mobile phase: V (methanol): V(0.004 mol/L KH2PO4 buffer, pH 4.5) = 50:50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of quality control. |
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| Keywords: | high performance liquid chromatography 6 aminopenicillanic acid penicillin G kalium phenylacetic acid benzylpenicilloic acid penicillin acylase penicillase |
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