用于识别不同细胞蛋白质组的噬菌体抗体芯片

将4个鼠源噬菌体抗体克隆和1个人源噬菌体抗体克隆偶联到羧基终止的硅片表面,制成分析型模型芯片.挑选健康人体淋巴细胞为正常细胞的代表, HeLa细胞为肿瘤细胞的代表,提取细胞的全部蛋白质并用荧光染料Cy3标记,与制成的分析芯片反应,得到了不同的结合图谱.实验结果表明,以噬菌体抗体为分子感受器的分析芯片可用于识别不同细胞的蛋白质组.

Phage Antibody Chip for Discriminating Proteomes from Different Cells

A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and 1 clone from human to the surface of carboxyl terminated silicon. The lymphocytes from healthy donors were selected as normal cells, while HeLa cells were selected as tumor cell. The whole proteins were labeled by fluorescent dye Cy3 after isolation from lymphocytes and HeLa cells, and then reacted with the analytical chip. A different binding pattern was obtained. Our results showed that the protein chip made of phage antibodies was able to recognize the proteome from different cells.