Estimation of PAMAM Dendrimers' Binding Capacity by Fluorescent Probe ANS

Dendrimers are globular, hyperbranched polymers which possess a high concentration of surface functional groups and internal cavities. These unique features make them very useful to many biomedical applications, especially as carrier molecules. This study presents results of estimation of polyamidoamine (PAMAM) dendrimers and human serum albumin (HSA) binding capacity of fluorescent probe 1-anilinonaphthalene-8-sulfonic acid (ANS). It has been shown that fluorescent probes can be used for quantitative analysis of dendrimers' binding capacity.