硫酸软骨素与天青A空间定向相互作用机理的研究

应用光谱法研究了天青A (AA)与硫酸软骨素 (CS)的相互作用吸收光谱 ,考察了不同AA CS摩尔比、乙醇、羟丙基 β 环糊精、TritonX 10 0以及NaCl等实验条件对AA与CS反应体系的影响。测得CS对AA的最大结合数N =15 1,结合常数K =5 2 4× 10 4 ,并且建立了AA与CS大分子空间定向相互作用的实验研究模型。实验结果表明 ,AA与CS相互作用产生的变色反应 ,不仅与AA与CS分子间的静电相互作用有关 ,而且还与CS大分子上结合态AA的密度有关。CS大分子上结合态AA分子间的疏水相互作用是形成5 5 0nm吸收峰以及产生变色反应的主要原因。

Spatial Orientation Interaction Mechanism between Chondroitin Sulfate and Azure A

The spatial orientation interaction mechanism of chondroitin sulfate (CS) and azure A (AA) was studied by spectrometry. The maximum binding number (N = 151) and the binding equilibrium constant (K = 5. 24 x 10(4)) were obtained. The molecular binding model of the interaction between AA and CS was established. The influence of the molar ratio of AA/CS, ethanol, hydroxypropyl-beta-cyclodextrin, triton X-100 and NaCl on the spectra of AA-CS complex was investigated. We conclude that the color change of complex depends on not only the electrostatic interaction between AA and CS but also the density of AA binding on CS. And the formation of the absorption peak at 550 nm and the color change of complex result mainly from the hydrophobic interaction between AA and AA binding on CS.