Separation of lansoprazole enantiomers in human serum by HPLC |
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Authors: | K. Borner E. Borner H. Lode |
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Affiliation: | 1. Instut für Klinische Chemie und Pathobiochemie, Freie Universit?t Berlin, Universit?tsklinikum Benjamin Franklin, 12200, Berlin, Germany 2. Stadtisches Krankenhaus Berlin Zehlendorf-Heckeshorn, Akademisches Krankenhaus der Freien Universit?t Berlin, Am Heckeshorn 33, 14109, Berlin, Germany
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Abstract: | Summary A new and simple HPLC method is described for the separation and quantitative determination of the (+)-and (−)-enantiomers of lansoprazole. The analytes were extracted from serum as previously described for whole lansoprazole [K. Borner, Chromatographia 45, 450–452 (1997)]. The enantiomers were separated by chromatography on a CHIRAL-AGPR column which contained covalently bound acid α1-glycoprotein as chiral selector. In the pure drug the (−)/(+) ratio was 0.99:1.01. In serum of twelve human volunteers the concentration of the (−)-enantiomer was 3 to 5 times higher than that of the (+)-enantiomer. Both enantiomers differ remarkably in their pharmacokinetics. |
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Keywords: | Column liquid chromatography Lansoprazole Enantiomers Human serum |
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