Use of a polycation spacer for noncovalent immobilization of albumin on thermally modified virus particles |
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Authors: | N A Nikitin A S Malinin A A Rakhnyanskaya E A Trifonova O V Karpova A A Yaroslavov J G Atabekov |
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Institution: | 1.Faculty of Biology,Moscow State University,Moscow,Russia;2.Faculty of Chemistry,Moscow State University,Moscow,Russia;3.Belozersky Institute of Physicochemical Biology,Moscow State University,Moscow,Russia |
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Abstract: | The noncovalent immobilization of the protein bovine serum albumin on the surface of spherical nanoparticles 330 ± 60 nm in
diameter is described. These nanoparticles are prepared by the thermal treatment of tobacco mosaic virus and are preliminarily
covered with a layer of the cationic polymer poly(N-ethyl-4-vinylpyridinium bromide). The electrostatic adsorption of the polycation on the surface of negatively charged spherical
nanoparticles (on average 1.2 × 104 macromolecules per particle) is accompanied by recharging of the surface; as a result, the negatively charged protein bovine
serum albumin can be adsorbed on it in an amount of 1.7 × 104 molecules per particle. The modification of spherical nanoparticles with the polycation and protein does not cause the aggregation
of particles. The spherical-nanoparticle-polycation-protein ternary complex demonstrates increased stability in salt solutions
relative to the spherical-nanoparticle-polycation binary complex. Because of the simplicity of the method used to modify the
surface of spherical nanoparticles, it shows promise for preparation of functionally active complexes. |
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