A computerised, unbiased method for epithelial measurement |
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| Authors: | Blomgren Bo Johannesson Ulrika Bohm-Starke Nina Falconer Christian Hilliges Marita |
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| Affiliation: | 1. Laboratorio de Ecotoxicología Acuática: Invertebrados Nativos (EAIN), Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Nuñez, 1428 Buenos Aires, Argentina;2. Laboratório de Mamíferos Aquáticos, Universidade Federal de Santa Catarina, SC, Brazil;3. Laboratorio de Ecosistemas Costeros, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia” (CONICET), Buenos Aires, Argentina;4. Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina;5. Universidad de Morón, Buenos Aires, Argentina;6. Programa de Pós-graduação em Ecologia, Departamento de Ecologia e Zoologia, Universidade Federal de Santa Catarina, SC, Brazil |
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| Abstract: | OBJECTIVE: To develop and evaluate a standardised method for unbiased measurements of epithelial thickness taking the variability of the dermal papillae in consideration. METHODS: A computer-assisted measurement program suitable for haematoxylin and eosin routine stained specimens has been developed. RESULTS: The developed program was designed to measure four different distance parameters, taking the number, height and width of dermal papillae into account. The measurement program gave very accurate results compared with manual measurements. The measurement results can be presented as tables or star graphs, and the results can be further processed by multivariate analysis. CONCLUSION: The computer-assisted measurement program is considered to be a valuable and reliable tool for measurements of epithelial thickness, irrespectively of the variability of the epithelial morphology. Since length, size and number of the papillae may change with certain pathological conditions, age and also under hormonal influence, this method can be a helpful diagnostic tool. |
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