| 光谱法研究三氯生与人类肿瘤相关DNA的相互作用 |
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| 引用本文： | 李莉,鲁嘉,刘雪梅,李卉卉,杨小弟.光谱法研究三氯生与人类肿瘤相关DNA的相互作用[J].分析测试学报,2012,31(8):951-956. |
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| 作者姓名： | 李莉 鲁嘉 刘雪梅 李卉卉 杨小弟 |
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| 作者单位： | 1. 江苏省新型动力电池重点实验室,江苏省生物功能材料重点实验室,南京师范大学化学与材料科学学院,江苏南京210097
2. 南京师范大学中国经济研究中心,江苏南京,210097 |
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| 基金项目： | 国家自然科学基金（20875047,20902048）；江苏省高校自然科学基金资助项目（09KJB150004）；江苏高校优势学科建设工程和水利部专项基金资助项目（201201018） |
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| 摘 要： | 采用紫外光谱、荧光光谱和圆二色谱等多种光谱方法研究了三氯生与人类肿瘤相关DNA(抑癌基因p53 DNA和癌基因C-myc DNA)的相互作用,以期为从分子水平上评价三氯生与人类肿瘤发生的危害性提供科学依据。紫外减色效应、KI猝灭受到抑制及圆二色谱正负峰强度减弱等结果表明三氯生以弱嵌插模式与两种DNA发生作用,三氯生与DNA的结合位点数均小于1,以及DNA解链温度小幅升高等结果证实三氯生部分嵌插入DNA的沟区,影响了DNA的双螺旋结构。
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| 关 键 词： | 三氯生 DNA 人类肿瘤 光谱法 相互作用 |
Study on the Interaction of Triclosan with Human Tumor Related DNA by Spectroscopic Methods |
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| LI Li
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LU Jia
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LIU Xue-mei
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LI Hui-hui
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YANG Xiao-di.Study on the Interaction of Triclosan with Human Tumor Related DNA by Spectroscopic Methods[J].Journal of Instrumental Analysis,2012,31(8):951-956. |
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| Authors: | LI Li LU Jia LIU Xue-mei LI Hui-hui YANG Xiao-di |
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| Institution: | 1(1.Jiangsu Key Laboratory of New Power Batteries,Jiangsu Key Laboratory of Biofunctional Materials,Department of Chemistry and Materials Science,Nanjing Normal University,Nanjing 210097,China;2.China Center for Economic Research,Nanjing Normal University,Nanjing 210097,China) |
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| Abstract: | Some investigations indicated that triclosan(TCS)has a genetic toxicity and would induce DNA damage.In this paper,a variety of spectroscopic methods,including UV spectrum,fluorescence spectrum and circular dichroism spectrum,were used to study the interaction of triclosan with human tumor related DNA(p53 DNA and C-myc DNA),aiming at providing the scientific basis for triclosan’s toxicity to human tumor at the molecular level.The UV experiments,KI quenching experiments and CD experiments suggested a weak intercalation between triclosan and DNA;Besides,melting experiments and fluorescence titration experiments also confirmed that triclosan interacts with DNA via partly interaction binding,thus affecting the double helix structure of DNA. |
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| Keywords: | triclosan DNA human tumor spectrometry interaction |
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